recombinant dengue ns1 128 antigens  (Native Antigen Inc)

Journal: Scientific Reports

Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

doi: 10.1038/s41598-026-35952-1

Figure Lengend Snippet: Detection of recombinant dengue NS1 antigen using Rabbit Polyclonal Antibodies against the Dengue NS1 antigen (RPAD). The ELISA plate was coated with rDNS1Ag (100 ng/well). To this, polyclonal antibodies raised in rabbits were added at different dilutions (1:50 to 1:50000 dilutions). The antibody response was detected by the secondary antibody, anti-rabbit-HRP (1:50000; 50 µl/well). Absorbance (OD) was measured at 452 nm. ns - statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

doi: 10.1038/s41598-026-35952-1

Figure Lengend Snippet: The RPAD is sensitive to detect all the four serotypes of dengue virus. The ELISA plate was coated with rDNS1Ag (100 ng/well) of different serotypes of DENV (1–4). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of polyclonal antibodies was detected by anti-rabbit-HRP. Absorbance (OD) was measured at 452 nm in an ELISA reader. D1 to D4 –NS1 antigen of Dengue virus serotypes, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

Techniques: Virus, Enzyme-linked Immunosorbent Assay, Binding Assay, Concentration Assay

Journal: Scientific Reports

Article Title: Evaluation of polyclonal antibodies raised in rabbits against dengue NS1 antigen

doi: 10.1038/s41598-026-35952-1

Figure Lengend Snippet: Evaluation of cross-reactivity of RPAD with the NS1 antigen of other Flaviviruses. The ELISA plate was coated with NS1 Ag of flavivirus (100 ng/well). To this, polyclonal antibody, RPAD (1:5000) was added. The binding of RPAD was detected by anti-rabbit-HRP. JE-Japanese Encephalitis Virus; TBEV Tick-borne Encephalitis Virus; WNV-West-Nile Virus; YFV-Yellow Fever Virus, D2 (R&D) – NS1 antigen used for immunizing the rabbits. ns- statistically not significant ( P > 0.05). A – Animal; D – concentration of rDNS1Ag.

Article Snippet: For developing the RPAD-ELISA, ELISA plates (Costar, Corning, USA) were coated with different concentrations of recombinant dengue NS1 antigens (DENV-1 to 4, Japanese encephalitis virus [JEV], tick-borne encephalitis virus [TBEV], West-Nile virus [WNV] and yellow fever virus [YFV]; The Native Antigen Company, Oxfordshire, UK: SKU: REC31778 –1781).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Virus, Concentration Assay

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Study design and results of serum levels of viral RNA and progesterone in ZIKV-infected pregnant marmosets. ( A ) The normal gestation period of the common marmoset is approximately 21 weeks (143–144 days) , and the period is shown as divided into three terms as a trimester every 7 weeks. The timing of infection and the end of pregnancy is indicated by the number of gestational weeks. Marmosets infected with ZIKV in the first (P-1 and -2) or second (P-3) trimester of pregnancy developed miscarriages, and a marmoset infected in the third trimester (P-4) gave birth to three early pups (N-1, -2, and -3); P-4 then became pregnant again and gave birth to a pup (N-4) (5 months after infection). P-5, infected with ZIKV in the first trimester, was euthanized 1 week after infection and submitted to histological analysis. ( B ) Concentrations of ZIKV RNA and progesterone in sera of ZIKV-infected pregnant marmosets. Black circles indicate the viral RNA concentrations and gray triangles indicate progesterone level. The gray areas on the left side of the graphs indicate pre-infection. Time points for miscarriage or delivery are indicated by arrows. In the case of P-1, the day of miscarriage was able to be accurately identified as day 19 post-infection by expulsion of the placenta. The lower limit of RNA quantitation (800 copies/mL) is indicated as horizontal lines. Samples less than the limit were calculated by extrapolation of the standard curve. ( C ) The serum collection schedule for the antiviral-neutralizing antibody test is shown. Sera were collected from ZIKV-infected pregnant marmoset P-4 and neonates N-1, -2, -3, and -4. Triangles indicate time points of serum collection; Sera were collected within 1 day of birth (N-1, -2, and -3) and at 4 months of age (N-4).

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Infection, Quantitation Assay

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Quantitation of ZIKV RNA in tissue samples derived from P1 and P-5 ^e

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Quantitation Assay, Derivative Assay

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: The levels of ZIKV RNA copies and TNFα in serum and amniotic fluid of P-5 ^a

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques:

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Trophoblast degeneration at the MFI of ZIKV-infected pregnant marmoset P-5. ( A ) Low magnification of MFI areas stained with HE; high magnification of significant cellular degeneration areas (black squares) stained with HE ( B ), anti-vimentin ( C ), anti-cytokeratin 7 (CK7) ( D ), and anti-CD31 ( E ) antibodies. Asterisks in ( B ) indicate degenerated cells.

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Infection, Staining

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Detection of ZIKV NS1 protein in the uterus: ( A ) uterine tissue from the infected pregnant marmoset P-5 stained with anti-NS1 antibody, shown at low magnification; MFI, decidua, BLE, and myometrium are shown as black squares; higher magnification images of these four areas are shown in ( B )–( E ), respectively. ( B ) MFI area stained with HE and anti-NS1 antibody. Asterisks indicate degenerated cells. ZIKV NS1 protein (green) and CK7 (red) were detected on the placental side of the MFI by double immunofluorescence. Regions 1 and 2, indicated by white squares, are shown at higher magnification. Uterine decidua ( C ), BLE ( D ), and myometrium ( E ) were stained with HE and anti-NS1 antibodies. Images of the BLE region of the uterus of a ZIKV-infected non-pregnant marmoset F-1 ( F ) and an uninfected non-pregnant marmoset ( G ) stained with anti-NS1 antibody are shown.

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Infection, Staining, Immunofluorescence

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Double immunofluorescence staining of ZIKV NS1-positive cells in the BLE region. Uterine tissue from ZIKV-infected pregnant marmoset P-5 was stained with a combination of anti-NS1 antibodies and antibodies against tissue components (i.e., anti-SMA, vimentin, or h-caldesmon antibody). The viral NS1 protein, target tissue proteins, and DNA are indicated by green, red, and blue fluorescence, respectively.

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Double Immunofluorescence Staining, Infection, Staining, Fluorescence

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Detection of inflammatory markers in uterine tissues. ( A ) Uterine tissue from ZIKV-infected pregnant marmoset P-5 was stained with an anti-caspase-3 antibody. Placental villi, MFI, decidua, and BLE are indicated by black squares, and high-magnification images of these areas are shown. Uterine tissues from ZIKV-infected non-pregnant marmoset F-1 ( B ) and uninfected non-pregnant marmoset ( C ) were stained with anti-caspase-3 antibody, showing areas of endometrium and BLE. ( D ) Uterine tissue from ZIKV-infected pregnant marmoset was stained with anti-MPO antibody, showing placenta, MFI, endometrium, and BLE regions. Similarly, ZIKV-infected non-pregnant marmoset F-1 ( E ) and uninfected non-pregnant marmoset ( F ) were stained with anti-MPO antibodies to reveal endometrium and BLE regions.

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Infection, Staining

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Detection of ZIKV NS1 and cleaved caspase-3 in ovaries. ( A ) Ovarian tissue from ZIKV-infected pregnant marmoset P-5 stained with anti-NS1 antibody is shown at low magnification. The follicle and corpus luteum, indicated by black squares, are stained with HE, anti-NS1, and anti-caspase-3 antibodies and are shown in higher magnification images. Ovarian tissues from ZIKV-infected non-pregnant marmoset F-2 ( B ) and uninfected non-pregnant marmoset ( C ) are stained with anti-NS1 and anti-caspase-3 antibodies, showing areas containing follicles and corpus luteum.

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: Infection, Staining

Journal: Microbiology Spectrum

Article Title: Pathological characterization of female reproductive organs prior to miscarriage induced by Zika virus infection in the pregnant common marmoset

doi: 10.1128/spectrum.02282-24

Figure Lengend Snippet: Anti-ZIKV neutralizing antibody titers in sera collected from dam P-4 and neonates ^a

Article Snippet: BALB/c mice were immunized with recombinant ZIKV NS1 protein produced in HEK293 cells (The Native Antigen Company, UK).

Techniques: